Figure 5 | Scientific Reports

Figure 5

From: Mitigation of total body irradiation-induced mortality and hematopoietic injury of mice by a thrombopoietin mimetic (JNJ-26366821)

Figure 5

Dose reduction factor and long term survival in C57BL/6 male mice. (A) DRF analysis of animals administered JNJ-26366821 at 1 mg/kg (■) or saline as a vehicle (□) 24 h post-TBI. Probit analysis was performed using IBM SPSS statistical analysis software and estimated survival was plotted as dashed lines (Saline) or Solid lines (TPOm). LD50/30 values were determined to be 7.78 Gy and 8.65 Gy for Saline and JNJ-26366821, respectively yielding a DRF value of 1.113. N = 24 mice per group. (B) 30 day and 6 month survival data for animals administered Saline (□) or JNJ-26366821 (■) 24 h after irradiation at 8.0 Gy. Kaplan–Meier survival curves were plotted using GraphPad software; n = 19–22 mice per group and the trend in survival was compared between vehicle and drug-treated groups (Log-rank test p < 0.0001). (C) 30 day and 6 month survival data for animals administered Saline (□) or JNJ-26366821 (■) 24 h after irradiation at 8.5 Gy. Kaplan–Meier survival curves were plotted using GraphPad software; n = 19–24 mice per group and trend in survival is compared between vehicle and drug-treated groups (Log-rank test p = 0.0003). Figures were plotted using GraphPad Prism 9 software V9.0.2; www.graphpad.com.

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