Figure 5
From: miR-96-5p targets PTEN to mediate sunitinib resistance in clear cell renal cell carcinoma
miR-96-5p directly represses PTEN expression. (a) Schematic representation of the predicted miR-96-5p target site sequence within the 3′ UTR of PTEN. Six nucleotides within the target site are complementary to the seed sequence of miR-96-5p. (b) Luciferase reporter assay. 293 T cells were co-transfected with luciferase reporters carrying either the wild-type PTEN 3′ UTR (PTEN WT) or the mutagenized PTEN 3′ UTR (PTEN Mut), as well as 50 nM negative control mimic (miR-Control) or miR-96-5p mimic. Data are presented as mean ± s.e.m. (n = 3). Significant results are presented as *P < 0.05.
