Figure 6
From: miR-96-5p targets PTEN to mediate sunitinib resistance in clear cell renal cell carcinoma
Downregulation of PTEN and upregulation of miR-96-5p are associated with sunitinib resistance in CCRCC cell lines. (a) Western blot (left) and quantification (right) of PTEN proteins in A498, TK10, and ACHN cells. GAPDH was used as a loading control. (b, c) The levels of PTEN mRNA (b) and miR-96-5p (c) were analyzed by qRT-PCR in A498, TK10, and ACHN cells. All data were normalized with GAPDH for mRNA or RNU6B for miRNA. (d, e) Dose-dependent effect of sunitinib on the viability (d) and death (e) of A498, TK10, and ACHN cells. Data are presented as mean ± s.e.m. (n = 3). Significant results are presented as *P < 0.05.
