Figure 7

miR-96-5p-mediated downregulation of PTEN regulates sensitivity to sunitinib in CCRCC cell lines. (a) The expression level of PTEN was downregulated after overexpression of miR-96-5p mimic (miR-96-5p) in the A498 cell line. (b, c) PTEN downregulation affects the viability (b) and death (c) of A498 cell lines upon treatment with 20 μM sunitinib. (d) Representative images of Matrigel invasion assay of NC and miR-96-5p transfected A498 cells under 10 uM sunitinib treatment (scale bars, 100 μm). The number of invaded cells was quantified and statistically analyzed (***P < 0.001). Error bars indicate the standard error of mean of four experiments. (e) The expression level of PTEN was upregulated after knockdown of miR-96-5p in the ACHN cell line. (f, g) The effects of PTEN upregulation on the viability (f) and death (g) upon 20 μM sunitinib treatment in ACHN. (h) The expression level of PTEN was verified after overexpression of PTEN in the miR-96-5p-expressing A498 cell line. (i) Upregulation of PTEN significantly sensitized cells to sunitinib treatment in miR-96-5p-expressing A498. Data are presented as mean ± s.e.m. (n = 3). Significant results are presented as *P < 0.05, **P < 0.01, ***P < 0.001. NC: negative control.