Table 1 Primer amplification characteristics of the target, marker, and endogenous genes for qRT-PCR in bread wheat.
From: Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat
Primer | Gene Bank accession number | Sequence information | Tm | GC% | Amplicon size (bp) | CDS sequence size (bp) |
|---|---|---|---|---|---|---|
TaARGOS-A | KX768731 | F: 5′-GATCATCTTCCACCACCATCTC-3′ | 60.72 | 50 | 117 | 2089 |
R: 5′-GCACCTACATGGGTGTTCTT-3′ | 57.51 | 50 | ||||
TaARGOS-B | KX768732 | F: 5′-GATTTGGAGGAGAGGGTGTTC-3′ | 59.93 | 55 | 120 | 2047 |
R: 5′-GCGTGGACAATCAAGCATAAG-3′ | 60.65 | 50 | ||||
TaARGOS-D | KX768733 | F: 5′-GCTGATCTGCACTCACCAAA-3′ | 59.99 | 50 | 156 | 2057 |
R: 5′-CAACCTTCTTGTCAGCAGCA-3′ | 60.18 | 50 | ||||
DREB3 | AY781349 | F: 5′-GGCATGCTGCAGTCTGATTA-3′ | 59.98 | 50 | 156 | 1325 |
R: 5′-AAGCCGACCAAACACCATAG-3′ | 59.99 | 50 | ||||
β-actin | AK457930.1 | F: 5′-GGAATCCATGAGACCACCTAC-3′ | 58.31 | 52 | 128 | 1527 |
R: 5′-GACCCAGACAACTCGCAAC-3′ | 59.25 | 58 |
