Figure 7

SET7 and ERRα are involved in cell invasion. (a) Co-immunoprecipitation of endogenous proteins in MDA-MB-231 cells with anti-SET7 or anti-ERRα antibodies and rabbit IgG used as a control. IP: immunoprecipitation, IB: immunoblotting. Proximity ligation assay (PLA) used to detect interaction of endogenous SET7 and ERRα in MDA-MB231 cells. Cells were counterstained with DAPI. See also Figure S5-c for PLA controls. (c) Indicated ERRα moiety were produced in vitro and identified on the left panel. Same ERRα moiety were hybridized on GST-SET7. (d) ERRα/SET7 regulated genes were analyzed by Gene Ontology (GO). After elimination of redundant terms, network of enriched GO terms obtained by REVIGO software (grouped according to semantic similarity) is shown. Colors indicate p-value. GO terms are coded by number (see Figure S6-a for correspondence with GO terms). (e) Confluent layers of MDA-MB-231 cells transfected with the indicated siRNA were scratch-wounded, phase contrast microphotographs were taken at the indicated times after wounding (left panels). Quantification (right panel) is displayed as percentage of remaining cell-free space at 12 h. Data are means of three independent experiments (3 fields per experiment) + /- sem. Significance was evaluated by t-test, with ***p < 0.005, *p < 0.05. Uncropped Western blot images are presented on Figure S9.