Figure 6
From: Multiple asters organize the yolk microtubule network during dclk2-GFP zebrafish epiboly
MT nucleation occurs at YSN centrosomes and YCL asters. γ-Tubulin expression can be observed through (A–C) immunostaining on fixed embryos and (D–F) in embryos coinjected with γ-tubulin-TdTomato mRNA and dclk2-gfp mRNA. γ-Tubulin signal has been found in (B) blastoderm centrosomes (blue arrow), (C) e-YSN centrosomes (red arrow) and (D–F) YCL asters colocalizing with dclk2-GFP signal (white arrows). In (A–C) the nuclei of both blastoderm and eYSN have been highlighted (yellow circles).
