Figure 4
Profiling proteins by inflammation response in airway epithelial cells. Inhibition of LPS and ATP-induced IL-1β secretion by PVP-I. Airway epithelial cells were treated with PVP-I (0.1%) for 1 h after LPS (1000 ng/mL) and ATP (5 mM) treatment. (a) After 24 h-incubation, the airway epithelial cell culture medium was assayed using an ELISA for IL-1β. Statistical significance: *P < 0.05 and **P < 0.01 compared with the control group; ##P < 0 .01 compared with the LPS/ATP group (b) After 24 h-induction, the pHNECs were subjected to cytokine antibody array assay. Each proteins represented by duplicate spots in the respective membrane. IL-1β protein is boxed in red. (c) Data were analyzed using the Quick Spots Image Analysis Software. Statistical significance: ***P < 0.001 compared with the control group; ##P < 0.01 compared with the LPS/ATP group.
