Table 1 Dilution sensitivity for 6-mm and 12-mm DBS eluted in EB and AD, tested with the standard and modified sample volume ARCHITECT anti-HCV assay.

From: Evaluation of hepatitis C virus antibody assay using dried blood spot samples

 

Plasma

12-mm DBS, STD assay, EB

12-mm DBS, STD assay, AD

12-mm DBS, MSV assay, EB

12-mm DBS, MSV assay, AD

6-mm DBS, MSV assay, EB

6-mm DBS, MSV assay, AD

DF

S/CO

S/CO

S/CO

S/CO

S/CO

S/CO

S/CO

Sample A

Neat

16

      

10

14.37

5.14

5.14

13.03

13.87

10.49

8.93

20

11.91

3.62

2.87

11.89

11.59

7.65

6.45

40

10.04

1.64

1.66

10.49

8.62

5.46

3.69

60

7.83

1.23

1.15

8.45

7.00

3.65

2.44

100

5.64

0.69

0.68

6.28

4.13

2.69

1.58

200

2.91

0.36

0.33

3.69

2.48

1.24

0.94

500

1.44

0.15

0.14

1.52

0.98

0.41

0.39

1000

0.91

0.09

0.08

0.78

0.53

0.31

0.21

Sample B

Neat

16.26

      

10

13.61

4.79

3.80

12.96

11.49

9.10

7.62

20

11.31

2.43

2.23

10.72

9.27

6.60

4.97

40

8.74

1.24

1.04

8.36

6.63

4.10

3.07

60

6.29

0.90

0.72

7.11

5.26

3.06

1.99

100

4.61

0.55

0.45

5.29

3.29

2.20

1.18

200

2.48

0.27

0.24

3.14

1.76

1.09

0.64

500

1.05

0.13

0.11

1.39

0.76

0.48

0.34

1000

0.42

0.08

0.08

0.73

0.49

0.24

0.18

  1. Each sample dilution was tested one time as 12 mm and 6 mm DBS eluted in elution buffer (EB) and assay diluent (AD) using anti-HCV standard and modified assay files. STD standard assay, MSV modified sample volume, EB elution buffer, AD assay diluent, S/CO signal to cutoff ratio. Italic values indicates reactive samples with S/CO ≥ 1.00.
Back to article page