Table 2 Precision of the modified sample volume ARCHITECT anti-HCV assay using whole blood DBS samples.

From: Evaluation of hepatitis C virus antibody assay using dried blood spot samples

Instrument

Run

DBS Rep

Neg whole blood

Sample A dilutions

D 1:500

D 1:200

D 1:100

D 1:20

Low Pos -1

Low Pos-2

Mid Pos

High Pos

1

1

1

0.09

2.11

4.15

6.69

12.10

1

1

2

0.09

2.23

4.16

6.56

11.60

1

1

3

0.04

1.63

4.17

6.57

12.28

1

2

1

0.10

2.03

4.28

6.61

11.50

1

2

2

0.09

1.79

3.33

5.42

11.23

1

2

3

0.09

1.84

4.31

6.32

11.27

1

3

1

0.08

1.39

4.07

6.58

11.98

1

3

2

0.09

1.95

3.99

6.59

8.44

1

3

3

0.10

2.03

4.33

4.84

11.15

Mean

  

0.09

1.89

4.09

6.24

11.28

SD

  

0.02

0.26

0.31

0.65

1.14

%CV

  

21.16

13.71

7.47

10.49

10.10

2

1

1

0.10

2.38

4.45

7.00

13.07

2

1

2

0.10

2.26

4.29

7.13

13.22

2

1

3

0.09

2.18

4.48

6.72

13.40

2

2

1

0.10

2.23

4.89

7.38

13.01

2

2

2

0.11

2.34

4.41

6.94

12.75

2

2

3

0.11

2.21

4.50

6.79

12.54

2

3

1

0.11

2.12

4.79

7.43

12.63

2

3

2

0.11

2.02

4.79

6.62

13.01

2

3

3

0.10

1.98

4.62

7.21

13.15

Mean

  

0.10

2.19

4.58

7.02

12.98

SD

  

0.01

0.13

0.20

0.29

0.28

CV%

  

6.84

6.11

4.45

4.07

2.18

Total

  

N = 18

    

Mean

  

0.09

2.04

4.33

6.63

12.13

SD

  

0.02

0.25

0.36

0.63

1.19

%CV

  

17.12

12.42

8.24

9.56

9.78

  1. SD standard deviation.
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