Figure 2
From: Single-tube collection and nucleic acid analysis of clinical samples for SARS-CoV-2 saliva testing
Effective SDS and Tween concentration determination for qPCR and virus inactivation. (A) Determination of the minimal Tween 20 and Tween 80 concentration necessary for SDS sequestration to enable amplification of 20,000 copies/µL of RNase P DNA. Ct cycle threshold, ND not detected. (B) Fluorescence assay of eGFP-expressing recombinant VSV-SARS-CoV-2 (107 pfu/mL) infecting Vero-E6 cells after incubation with 0.1% (w/v) SDS for 15–120 s. Only a sample that was not exposed to SDS showed infectivity. 15 s exposure to 0.1% (w/v) SDS proved sufficient to completely inactivate the virus, likely due to dissolution of its membrane and denaturation of the sample proteins.
