Figure 3

Comparison of standard qPCR to SDS/Tween and SDS/Tween agarose reactions. (A) Comparison of 10 µL qPCR reactions for SARS-CoV-2 N1 gene DNA amplification, containing 3% (v/v) of Tween 80 with (R² = 0.9781) and without 0.01% SDS (w/v, final; R² = 0.9818) and 1% (w/v) agarose (R² = 0.9260). (B) Comparison of a 10 µL qPCR reaction for SARS-CoV-2 N1 gene DNA containing 3% (v/v) Tween 80 (R² = 0.9908) with a 5 µL qPCR reaction (R² = 0.8451) for SARS-CoV-2 N1 DNA detection, containing 3% (v/v) Tween 80, 0.01% SDS (w/v, final) and 1% (w/v) agarose. In all conditions containing SDS, the DNA samples were initially incubated with 0.1% SDS (w/v) in Tris/EDTA buffer to simulate denaturing conditions for clinical samples. Ct cycle threshold.