Figure 2
taken from 1 donor. Graph shown in (A) shows percentages of granulocytes and agranulocytes for 4 donor, analysed using one-way repeated measures ANOVA (*p < 0.5, **p < 0.01). Graphs shown in (B), (D), and (F) show data from 6 donors paired for the 4 h and 48 h results (lines join results from a single individual), and analysed using Wilcoxon matched-pairs signed rank test (*p < 0.5).
Cell population 4 and 48 h post-intradermal injection of killed S. pnuemoniae in the forearms of healthy volunteers. Data were obtained by flow cytometry of blister fluid obtained 4 and 48 h after intradermal inoculation of 7.5 × 105 CFUs of UV-killed TIGR4 S. pnuemoniae. (A) Proportions and (B) numbers of granulocytes and agranulocytes in 4 and 48 h blister fluid samples as identified by forward and side scatter. (C) to (E) Analysis of the agranulocytes population using flow cytometry for CD3 surface expression.Proportion (C) an numbers (D) of CD3 negative agranulocytes. Proportions (E) and numbers (F) of CD3 positive agranulocytes. (A), (C), and (E) show representative flow cytometry plots from blood and blister fluid samples
