Figure 3

Lymphocyte subpopulations 4 and 48 h post-intradermal injection of killed S. pnuemoniae in the forearms of healthy volunteers. Data were obtained using flow cytometry to measure expression of lymphocyte cell surface markers in blister fluid cells obtained 4 and 48 h after intradermalin inoculation of 7.5 × 10⁵ CFUs of UV-killed TIGR4 S. pnuemoniae. (A) to (B) representative flow cytometry plots of CD4/CD8 (A) and CD25⁺CD127 (B) populations in blood and blister fluid samples taken from 1 donar. (C) Total cell number of CD3⁺, CD4⁺, CD8⁺, CD25⁺CD27⁻, and CD4⁻CD8⁻ cells in 4 (left column each cell type, circle symbols) and 48 (right column each cell type , square symbols) hour blister fluid samples. (D) Proprtion of CD3⁺ cells that were CD4⁺ (circle symbols), CD8⁺ (square sumbols), CD4⁻CD8⁻ (triangle symbols) in pre-infection blood samples, and 4 and 48 h blister fluid. For (C) and (D) each symbols represents data from an individuals volunteers, columns represent means, and error bars SDs. (E), (F), and (G) cell numbers per ml of blister fluid for CD4⁺ (E), CD8⁺ (F), and CD25⁺CD27⁻ (G) subsets for 6 donors paired for the 4 h anf 48 h results (lines join results from a single individual), and analysed using Wilcoxon matched-pairs signed rank test (*p < 0.5).