Figure 1
From: Activation of lysosomal mediated cell death in the course of autophagy by mTORC1 inhibitor
SDS-203 decreases cell viability by the non-apoptotic way in pancreatic cancer cell line MIA PaCa-2. (A–D) MIA PaCa-2 cells were incubated with different concentrations of SDS-203 (5, 10, 20, 30 µM) for 24 h or single concentration (20 µM) for various time points (0, 6, 12, 24 h) then cells were stained with Annexine-V-FITC and PI (1 µg/mL) followed by quantification of apoptotic cells by flowcytometry (E) MIA PaCa-2 cells were treated with SDS-203 (20 μM) in a time-dependent manner (0, 12, 24, 36 h) and protein expression of cleaved PARP1 and cleaved Caspase3 were determined by western blotting, camptothecin (1 µM) was taken as a positive control. Each experiment was repeated three times. p-value represents: **p < 0.01; ***p < 0.001vs. control.
