Figure 3
From: Activation of lysosomal mediated cell death in the course of autophagy by mTORC1 inhibitor
SDS-203 induces autophagy by inhibiting mTORC1 activity. (A, B) MIA PaCa-2 cells were treated with SDS-203 (20 μM) for various time points (0, 1, 2, 3, 6, 12, 24, 36 h) and its effect on p-mTORC1 along with its downstream target s– p-p70S6kinase and p-4EBP1, were analyzed and quantified. (C, D) MIA PaCa-2 cells were incubated with SDS-203 (20 µM) for 24 h in the presence or absence of IGF-1 (50 ng) or l-leucine (0.5 mM). Protein expression was analyzed and quantified by using ImageJ software. (E) Graphical representation (MTT) of survival percentage of MIA PaCa-2 cells treated with SDS-203 (20 µM) for 48 h in presence or absence of l-leucine (0.5 mM) or IGF-1 (50 ng). All data are presented as the mean of SD from three independent experiments. Statistics, Student’s t test *p < 0.05, **p < 0.01, ***p<0.001 vs. control (0.1% DMSO).
