Figure 5
From: Activation of lysosomal mediated cell death in the course of autophagy by mTORC1 inhibitor
SDS-203 induces lysosomal biogenesis in MIA PaCa-2 cells. (A, B) Represent confocal images and puncta density of LAMP1expression upon treatment of SDS-203 (20 µM) for 6, 12, 24 h or rapamycin (200 nM) for 12 h. (C, D) Demonstrates LAMP1 protein expression (Western botting) and quantification in MIA PaCa-2 cells incubated with SDS-203 (20 µM) for different time points. (E, F) Demonstrate respective lysotracker DND-99 probe (50 nM) staining and quantification of fluorescent signal in MIA PaCa-2 cell treated with SDS-203 (20 µM) for different time points. (G) Represents the accumulation of unfused autophagosomes and lysosomes in pancreatic cancer cells upon SDS-203 treatment versus autolysosomes formation in rapamycin-treated samples, visualized under electron microscopy. Statistics, Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001 vs. control (0.1%DMSO).
