Figure 6
From: Activation of lysosomal mediated cell death in the course of autophagy by mTORC1 inhibitor
SDS-203 participates in lysosomes and autophagosome fusion inhibitor. (A) MIA PaCa-2 cells treated with SDS-203 prior to fluorescent antibody labeling against endogenous LAMP1 (green) and mTORC1 (red) colocalization (yellow). (B) Shows treatment colocalization correlation compared to control. (C) Pancreatic cancer cells were treated with SDS-203 (0, 10, 20 µM) and taken for mTORC1 and LAMP1 interaction investigation by immunoprecipitation (ip immune precipitation, WCL whole cell lysate). (D, E) Represents the expression of mTORC1 in vector or WT mTORC1 plasmid transfected cells and its quantification respectively. (F) SDS-203 treated to mTORC1 overexpressed MIA PaCa-2 cells and expression of LC3-II and LAMP1 were detected. (G) Densitometry of blots. Each experiment was repeated thrice, ip experimentation was done twice, and the present data represent the mean of three individual experiments, taken as ± SEM, where **or##p < 0.01 and ***or###p < 0.001 vs. control.
