Table 4 Details on primary and secondary antibodies used in immunocytochemistry.
Raised against | Code no | Host | Working dilution | Supplier |
|---|---|---|---|---|
CD3 | 1127 | Goat | 1:1,000 | Santa Cruz Biotechnology Inc., CA, USA |
Proinsulin | 9004 | Guinea pig | 1:2,000 | Eurodiagnostica AB, Malmö, Sweden |
Glucagon | 7810 | Rabbit | 1:2,000 | Eurodiagnostica AB, Malmö, Sweden |
Glucagon-like peptide-1 | A2 | Rabbit | 1:4,000 | Kind gift from Prof R Ekman, Gothenburg, Sweden |
Somatostatin | 1758 | Rabbit | 1:1,600 | Kind gift from Prof JJ Holst, Copenhagen, Denmark |
Nkx6.1 | NBP1-49,672 | Rabbit | 1:1,600 | Novusbio, Abingdon, Oxford, UK |
PDX-1/IPF1 | AF2517 | Goat | 1:400 | R&D Systems, Abingdgon, Oxford, UK |
Guinea pig IgG | 706 485 148 | Donkey | 1:1,500 | Jackson ImmunoResearch, Europe Ltd, Sweden |
Rabbit IgG | 711 585 152 | Donkey | 1:1,500 | Jackson ImmunoResearch, Europe Ltd, Sweden |
Goat IgG | 705 545 147 | Donkey | 1:1,200 | Jackson ImmunoResearch, Europe Ltd, Sweden |
