Figure 1 | Scientific Reports

Figure 1

From: Adiponectin accumulation in the retinal vascular endothelium and its possible role in preventing early diabetic microvascular damage

Figure 1

Localization of adiponectin in the mouse retina. (A, B) Representative images of retinal whole-mount immunofluorescence staining of male wild-type (WT), adiponectin knockout (APN-KO), and T-cadherin knockout (Tcad-KO) mice. (A) Triple immunostaining for APN (red), CD31 (green), and αSMA (gray) in the primary layer. Scale bar = 100 μm. (B) Triple immunostaining for APN (red), CD31 (green), and NG2 (gray) in the capillary layer. Scale bar = 100 μm. (C) Immunoblots for APN, Tcad, and β-actin proteins extracted from whole retinas of WT, APN-KO, and Tcad-KO mice. Original blots/gels are presented in Supple. Fig. 1. (D) Representative images of triple immunostaining for APN (red), CD31 (green), and αSMA (gray) of retinas from WT mice. Scale bar = 100 μm. (E, F) High magnification images of the arteriole (E) and the transitional zone between the arteriole and the capillary (F). Scale bar = 25 μm.

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