Figure 4 | Scientific Reports

Figure 4

From: Adiponectin accumulation in the retinal vascular endothelium and its possible role in preventing early diabetic microvascular damage

Figure 4

Glucose-lowering effects of dapagliflozin on vascular cellular adhesion molecule-1 and claudin-5 expression in the retinal vascular endothelium. After the onset of streptozotocin (STZ)-induced diabetes, 8-week-old male wild-type (WT) mice were treated with vehicle (STZ/Vehi) or dapagliflozin (STZ/Dapa) for 6 weeks. Mice without STZ injection were also analyzed as a control (Cont). Immunofluorescence staining was conducted using retinal whole-mount from each mouse. (A) Representative immunofluorescence images for vascular cellular adhesion molecule-1 (VCAM-1) (red) and isolectin B4 (IB4) (gray). Scale bar = 100 μm. (B) The % area of the VCAM-1-positive region in IB4-positive endothelium. Quantification was performed using two randomly obtained sections for each mouse. n = 4 for Cont, n = 7 for STZ/Vehi, and n = 6 for STZ/Dapa. Data are the mean ± SEM; **P < 0.01. N.S., not significant (one-way ANOVA with Tukey’s post hoc test). (C) Representative immunofluorescence images for claudin-5 (green) and CD31 (gray). Scale bar = 100 μm.

Back to article page