Table 2 Summary of the de novo homology matching performed on the results from the proteomic analysis of the Palmyra samples. Accession codes are in the format of UniProtKB.

From: Species identification of silks by protein mass spectrometry reveals evidence of wild silk use in antiquity

Sample

Highest scoring peptide

Accession

−10lgP

Cov

(%)

#Peptide

(unique)

Unique peptides (position, −10lgP)

A

S8

warp

SA12GSGAGGRGD

(−10lgP = 47.33)

Antheraea specific

Q8ISB3|ANTMY

163.41

30

31 (12)

SGA12SGAGGR (243–263, 46.77)

SSA11SAAR (484–500, 44.12)

E1CGA3|ANTYA

160.30

7

35(7)

 

O76786|ANTPE

151.28

7

31(3)

 

A0A0K0KR73|ANTASA

121.62

8

18(7)

 

B

S8

weft

GGYGSGSSA11SAAR

(−10lgP = 53.66)

A. mylitta specific

Q8ISB3|ANTMY

238.35

48

48 (29)

GGYGSGSSA11SAAR (478–500, 70.75)

SA14GSGAGGRGDGGY (206–232, 41.95)

GA12SGAGGR (244–263, 40.40)

GGGGFYETHDSYSSY (134–148, 37.28)

GSDSA13GSGAGGVGGGYGR (346–375, 31.11)

SA15SGAGGR (176–197, 27.45)

NIHHDEYVDSHGQLVER (20–36, 21.13)

  

E1CGA3|ANTYA

197.23

11

44 (14)

 

O76786|ANTPE

195.64

10

36 (6)

 
  

A0A0K0KR73|ANTASA

156.27

8

16 (8)

 

C

S48

GAGSA10GAGASR

(−10lgP = 49.64)

A. pernyi specific

O76786|ANTPE

131.54

8

12 (5)

 
  

1CGA3|ANTYA

123.78

8

14(6)

 

Q8ISB3|ANTMY

115.17

22

12(7)

SA15SGAGGR (176–197, 39.04)

SGA12SGAGGRGD (243–265, 37.66)

SGSSA11SAAR (482–500, 36.05)

D

S49

T1

GYGSGSSA10SAAR

(−10lgP = 55.54)

A. pernyi specific

Q8ISB3|ANTMY

179.20

36

17 (13)

GSGSSA11SAAR (481–500, 64.48)

QASHGAGGAAGAAAGAAASSSVR (111–133, 44.75)

SGA12SGAGGR (243–263, 25.96)

GQATVVMDGAMAAMVLTR (297–314, 20.53)

  

E1CGA3|ANTYA

172.99

5

16(4)

 

O76786|ANTPE

170.24

6

18(5)

 

E

S49

T2

SA11SAAR

(−10lgP = 32.85)

A. mylitta specific

E1CGA3|ANTYA

116.35

7

25(8)

 
  

A0A0K0KR73|ANTASA

105.68

5

19(2)

 

Q8ISB3|ANTMY

103.05

24

15(3)

SA11SAAR (485–500, 32.35)

GSDPGA13 (452–469, 24.41)

  1. The −10lgP score indicates the statistical significance of the peptide-spectrum match. Peptides were filtered by −10lgP values ≥ 20, and the values ≥ 70 is usually considered significant for proteins.
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