Table 2 MIC, MHC and TI of LfcinB6, KR-12-a4 and Lf-KR against different bacterial strains.

From: A novel hybrid peptide composed of LfcinB6 and KR-12-a4 with enhanced antimicrobial, anti-inflammatory and anti-biofilm activities

Bacterial strains

MICa (μM)

LfcinB6

KR-12-a4

Lf-KR

Melittin

Gram-positive bacteria

S. aureus (KCTC 1621)

 > 64

16

4

4

S. epidermidis (KCTC 1917)

 > 64

16

8

4

B. subtilis (KCTC 3068)

 > 64

4

4

4

Resistant Gram (+) bacteria

MRSAe (CCARM 3089)

 > 64

64

8

4

MRSA (CCARM 3090)

 > 64

 > 64

8

4

MRSA (CCARM 3095)

 > 64

 > 64

8

8

VREFf (ATCC 51,559)

 > 64

64

8

4

Gram-negative bacteria

E. coli (KCTC 1682)

 > 64

16

8

4

P. aeruginosa (KCTC 1637)

 > 64

8

8

16

S. typhimurium (KCTC 1926)

 > 64

8

4

4

Resistant Gram (−) bacteria

MDRPAg (CCARM 2095)

 > 64

4

8

8

MDRPA (CCARM 2109)

 > 64

64

8

8

GMb (μM)

 > 64

43.3

7.0

6.0

MHCc (μM)

 > 128

 > 128

 > 128

5.0

TId (MHC/GM)

0.5

5.9

36.6

0.8

  1. aMinimum inhibitory concentrations (MICs) were determined as the lowest concentration of the peptides that inhibited bacterial growth.
  2. bThe geometric mean (GM) of the peptide MICs against the tested bacterial strains was calculated. When no antimicrobial activity was observed at 64 μM, a value of 128 μM was used to calculate the therapeutic index.
  3. cMHC is the minimum hemolytic concentration that causes 10% hemolysis of sheep red blood cells (sRBCs). When no detectable hemolytic.
  4. activity was observed at 128 μM, a value of 256 μM was used to calculate the therapeutic index.
  5. dTherapeutic index (TI) is the ratio of MHC to GM. Larger values indicate greater cell selectivity.
  6. eMRSA: methicillin-resistant Staphylococcus aureus.
  7. fVREF: vancomycin-resistant Enterococcus faecium.
  8. gMDRPA: multidrug-resistant Pseudomonas aeruginosa.
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