Figure 2 | Scientific Reports

Figure 2

From: Identification of macrophages in normal and injured mouse tissues using reporter lines and antibodies

Figure 2

Identification of liver macrophages using antibodies and reporter lines. (A–C) Dual labeling for GFP and antibodies to Mac2 (A), F4/80 (B) and lysozyme M (C,D). Arrows show double positive cells. Quantitative analysis shows that Mac2 (A) and F4/80 (B) co-localize with CSF1R+ cells. In contrast, the majority of CSF1R+ cells are not labeled with the rabbit monoclonal anti-lysozyme antibody EPR2994(2) (EPR, C arrowheads). In order to examine whether the lack of lysozyme immunoreactivity in the majority of hepatic macrophages reflects the low sensitivity of the antibody, we also tested the rabbit polyclonal anti-lysozyme antibody NBP2-61,118 (NB, D). Quantitative analysis showed that virtually all hepatic macrophages exhibited lysozyme immunoreactivity for the NBP2-61,118 antibody. (E) A representative image shows that the antibody to Mac3 is not suitable for identification of hepatic macrophages, exhibiting punctate staining throughout the liver. (F–H) CX3CR1/Mac2, CX3CR1/F4-80 and CX3CR1/Lysozyme staining confirm the absence of CX3CR1 in hepatic macrophages. Scale bar: 20 μm.

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