Figure 6 | Scientific Reports

Figure 6

From: Identification of macrophages in normal and injured mouse tissues using reporter lines and antibodies

Figure 6

Identification of macrophages in the normal heart using CSF1R-EGFP reporter line and macrophage antibodies. Dual fluorescence for GFP and macrophage antibodies in heart sections from CSF1R-EGFP mice. CSF1R+ cells are sparsely distributed throughout the left ventricle (LV), and left atrium (LA), but exhibit a higher density in mitral valve leaflets. Only a small fraction of CSF1R+ cells express Mac2 (A–C), Mac3 (D–F), or LyzM (stained with anti-lysozyme antibody, clone EPR2994(2)) (G–I) (arrows). F4-80 (ab111101) does not stain any myocardial cells (J). Scale bar: 20 μm.

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