Table 2 Patterns or macrophage labeling in formalin-fixed paraffin-embedded mouse organs using reporter lines and antibodies.
Organ | CSF1REGFP (reporter) | CX3CR1EGFP (reporter) | Mac2 (IR) | Mac3 (IR) | F4/80 (IR) | Lysozyme (IR) |
|---|---|---|---|---|---|---|
Liver | Abundant CSF1R+ cells | No detectable staining | Labels all CSF1R+ cells | Diffuse staining in liver parenchyma | Labels all CSF1R+ cells | Staining is dependent on the antibody used. Ab NBP2-61,118 labels all hepatic macrophages, whereas Aby EPR2994(2) stains a subset of CSF1R+ cells |
Spleen (RP) | Abundant CSF1R+ cells | Smaller population of CX3CR1+ cells that is not labeled with anti-macrophage antibodies | Labels a subset of CSF1R+ cells | Labels the majority of CSF1R+ cells | Labels the majority of CSF1R+ cells | Labels a subset of CSF1R+ cells |
Spleen (WP) | Low density of CSF1R+ cells | Abundant CX3CR1+ cells | Labels the majority of CSF1R+ cells and the majority of CX3CR1+ cells | Labels a subset of CSF1R+ cells and the majority of CX3CR1+ cells | Labels a subset of CSF1R+ , but not CX3CR1+ cells | Labels a subset of CSF1R+ cells and the majority of CX3CR1+ cells |
Kidney (TI) | Low density of CSF1R+ cells | Low density of CX3CR1+ cells | Labels ~ 50% of CSF1R+ cells and a small subset of CX3CR1+ cells. Intensely stains tubular epithelial cells | Labels ~ 50% of CSF1R+ cells and a small subset of CX3CR1+ cells. Stains many tubular epithelial cells | BM8 and CI:A3-1 clones, but not the recombinant monoclonal ab111101, label renal interstitial macrophages | Does not stain CSF1R+ and CX3CR1+ cells. Stains the luminal surface of many tubular epithelial cells |
Kidney (Gl) | Very few CSF1R+ cells | Very few CX3CR1+ cells | Labels the majority of CSF1R+ and CX3CR1+ cells | No detectable staining | BM8 and CI:A3-1 clones, but not ab111101, label glomerular macrophages | No detectable staining |
Lung | Large population of CSF1R+ cells | Significant population of CX3CR1+ cells | Labels the majority of CSF1R+ cells, but a small subset of CX3CR1+ cells | Labels the majority of CSF1R+ cells, but a small subset of CX3CR1+ cells | Labels a large population of CSF1R- and CX3CR1-negative cells | Labels the majority of CSF1R+ cells, but a small subset of CX3CR1+ cells |
Heart (N) | Small population of CSF1R+ cells | Small population of CX3CR1+ cells | Labels a subset of CSF1R+ and CX3CR1+ cells | Labels subsets of CSF1R+ and CX3CR1+ cells | No detectable staining of CSF1R+ and CX3CR1+ cells with F4/80 antibodies | Labels a subset of CSF1R+ and CX3CR1+ cells |
Heart (MI) | CSF1R+ cell density increases 24 h after infarction (early inflammatory phase), and peaks 7 days after MI (proliferative phase) | Density of CX3CR1+ cells in the infarct is much lower than the number of CSFR1+ cells at all timepoints, and peaked 7 days after MI | Labels virtually all CSF1R+ cells during the proliferative phase. Labels the majority of CSF1R+ cells during the inflammatory and maturation phase | Labels most CSF1R+ cells during the proliferative and maturation phase. Labels the majority of CSF1R+ cells during the inflammatory phase | F4/80 antibodies do not label the vast majority of infarct macrophages | Labels the majority of CSF1R+ cells during the inflammatory phase. A CSF1R+ /LyzM-population emerges during the proliferative phase |
