Figure 1
From: Gut-derived butyrate suppresses ocular surface inflammation
C57BL/6 J mice express mRNA and protein for the SCFA transporter SLC5A8 on the ocular surface. (A) Quantitative digital PCR was used for amplification of cDNA prepared from wild-type C57BL/6 murine corneal epithelium and conjunctival tissue. Each datapoint represents tissue from one animal. The number of transcripts detected was normalized to the concentration of DNA. (B) Cornea and conjunctival (Conj) sections from wild-type (WT) C57BL/6 and Slc5a8 knockout (KO) mice were stained with H&E to assess morphology. Representative images are shown. (C) Cornea and conjunctival (Conj) sections from wild-type (WT) C57BL/6 and Slc5a8 knockout mice were immunostained with anti-mouse SLC5A8 antibody using chromogenic detection (reddish-brown). Nuclei were stained with hematoxylin (purple). Slides stained with anti-rabbit IgG are shown as a negative control. Representative images are shown. CO = cornea; CJ = conjunctiva.
