Figure 7
From: Sexual dimorphic impacts of systemic vincristine on lower urinary tract function
Tubb3 immunofluorescence labeling. (A) representative immunofluorescent labeling images with antibody against Tubb3 (red) of the bladder sections from ChAT-Cre+/−:: ZsGreen+/− mice, in which the expression of ZsGreen protein (green) were restricted in ChAT-expression cells. The merged images of ZsGreen and Tubb3 IR (right), shows Tubb3 IR in the Zs-Green positive cells in cluster forms [N = 3] (B), representative images of Tubb3 IR (red) in the bladders from female (upper) and male (lower) from the control (left) and VCR (right) groups. Cell nuclei were stained with DAPI (blue) [N = 4–5 per group per sex]. Scale bars = 20 µm (A) and 250 µm (B). The area (C) and mean intensity (D) measurements for Tubb3-IR in the bladder sections. *p < 0.05, **p < 0.005. Figures were prepared using Adobe Photoshop CS6 and GraphPad Prism 8.4.3 (https://www.graphpad.com/scientific-software/prism/).
