Figure 6
Upregulation of coagulation in thoracic lymph from CS-exposed mice. View of IPA-predicted prothrombin activation pathway (A) and coagulation system (B) in lymph from CS-exposed mice compared to room air mice. The experimental log ratio of proteins in lymph from CS-exposed versus room air mice are indicated. Experimentally determined proteins that were upregulated are depicted in red, and those that were downregulated are shown in green. Proteins that were predicted by IPA to be inhibited due to their networking relationship with the experimentally determined proteins are shown in blue, and proteins that were predicted to be activated are shown orange. IPA predicted enhanced coagulation in the lymph from mice exposed to smoke as compared with room air, mainly due to the increased in the abundance of Factors 5/5A, alpha-thrombin, SERPINSF2, C1, A1 and Kininogen 1 (shown in red). Accordingly, IPA predicted an increased in the activation of other coagulation factors like FVIIIa, F3, F10AF7a, FXIa, FIXa and F13A1 (orange). IPA also predicted a decrease in plasmin activity (blue). Results from 2 independent experiments with a total of n = 10 CS-exposed mice and n = 12 room air mice.
