Figure 1

Outputs of ddPCR and qPCR of CNV based sex detection using markers LdVssc and LdUBE3B typically detected in female (left side) and male (right side). Counts of positive and negative droplets (A, B), qPCR Ct values (C, D), qPCR melting analysis (E, F), and electrophoretograms of LdVssc and LdUBE3B amplicons (G, H).