Table 1 Markers designed for CNV-based sex identification system in CPB.

From: Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation

Gene

Primer

Sequence (5′–3′)

Sequence

Primer position

Amplicon size (bp)

LdVssc

LdVssc-F

AGAATCATGGATTGTCCGAAGGTT

Ldec_2.0 scaffold2248 (NW_019291534.1)

14,058–14,081

243

LdVssc-R

GAGGGTGGTAAGAGTGGCAAAAGT

14,277–14,300

LdUBE3B

LdUBE3B-F

AACAACTGCAGCATCTGAAACTCC

Ldec_2.0 scaffold202 (NW_019289582.1)

72,367–72,390

250

LdUBE3B-R

TACGGCTTTGAACACTTTGACACA

72,593–72,616

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