Table 2 Summary of results of ddPCR and qPCR in the collection A.
Target (LdVssc gene) | Reference (LdUBE3B gene) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Females | Males | Females | Males | |||||||||||||
Min | Max | \(\overline{{\text x}}\) | SD | Min | Max | \(\overline{{\text x}}\) | SD | Min | Max | \(\overline{{\text x}}\) | SD | Min | Max | \(\overline{{\text x}}\) | SD | |
ddPCR assay | ||||||||||||||||
Number of positive droplets | 6999 | 7058 | 7011 | 281 | 3405 | 3491 | 3457 | 138 | 6981 | 7051 | 7021 | 279 | 6821 | 6990 | 6914 | 281 |
Number of negative droplets | 10,653 | 10,801 | 10,787 | 438 | 14,103 | 14,295 | 14,193 | 554 | 10,636 | 10,795 | 10,704 | 431 | 10,715 | 10,873 | 10,784 | 427 |
Number of template copies per 1 μL | 623 | 635 | 627 | 26 | 249 | 263 | 259 | 12 | 621 | 632 | 624 | 24 | 499 | 521 | 509 | 19 |
qRT-PCR assay | ||||||||||||||||
Ct | 20.93 | 22.12 | 21.43 | 1.11 | 22.86 | 24.12 | 23.51 | 1.19 | 20.81 | 22.08 | 21.51 | 1.13 | 20.95 | 22.14 | 21.40 | 1.10 |
