Table 2 Calculations of sensitivity, specificity, PPV, NPV and accuracy for tissue-of-origin tests.

From: A high-throughput real-time PCR tissue-of-origin test to distinguish blood from lymphoblastoid cell line DNA for (epi)genomic studies

Tissue-of-origin assay

Sensitivity (%)

Specificity (%)

PPV (%)

NPV (%)

Accuracy

Ct GAPDH/Ct EBV

98.5

100.0

100.0

97.8

0.991

Ct GAPDH/Ct TCRγ

94.3

99.4

99.5

92.4

0.962

Tm TCRβ

98.2

98.7

99.1

97.5

0.984

Combined EBV, TCRγ and TCRβ tests

98.5

100.0

100.0

97.8

0.991

  1. For our calculations, blood + was considered as the positive result, and LCL + and uncertain origin as negatives results.
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