Figure 1
From: Assessment of immunogenicity and drug activity in patient sera by flow-induced dispersion analysis
Schematics showing ADA classification as function of apparent hydrodynamic radius (Rh) of TNF-α-AF488 (indicator) and adalimumab (analyte). TNF-α-AF488 is illustrated as a soluble homotrimer (blue) conjugated to a fluorophore (green) which enables selective Rh measurements via fluorescence detection. Adalimumab is exemplified as an antibody (bordeaux) targeted by non-neutralizing antibodies (orange) and neutralizing antibodies (black). The yellow ruler illustrates the link between Rh, normal drug activity (left), and presence of ADAs (middle and right). Created by author Morten E. Pedersen using biorender.com.
