Figure 2

Zn{[CH3)3C]2Sal}22− inhibits 4T1 cell migration and invasion. (A) With 10 and 25 μM Zn{[CH3)3C]2Sal}22− treatment, 4T1 cell migration ability was determined by wound healing assay at 0 h, 24 h, 48 h, and 72 h. (B) Statistical analysis of the wound healing assay of three independent experiments. Error bars represent the mean ± SEM, ***P < 0.001. C) The invasion ability was determined by a Transwell invasion assay after 22 h incubation. (D) Statistical analysis was performed with three independent Transwell invasion assays. Error bars represent mean ± SEM. ***P < 0.001.