Figure 4
Zn{[CH3)3C]2Sal}22− inhibits the activation of the STAT3 signaling pathway and SRC pathway. (A) The protein expression levels of STAT3, p-STAT3, p-Src, and Src at different time points in 4T1 cells treated with Zn{[CH3)3C]2Sal}22− were analyzed by western blot. (B) The protein expression levels of Akt and p-Akt were analyzed by western blot after 30 min of treatment with Zn{[CH3)3C]2Sal}22− or MK2206 alone or in combination. (C–E) Expression changes (fold change relative to control) of p-STAT3, p-Src and p-Akt473 were analyzed by densitometry with the results of two independent Western blots. Data are presented as the mean ± SEM (*P < 0.05, **P < 0.01).
