Figure 2
Ran was mislocalised to the cytoplasm in cells expressing DPRs. (A–C) HeLa cells transfected with GFP-tagged DPR constructs or empty pEGFP-N1 vector as a GFP-only control were fixed and stained 48 h post-transfection. Ran (red) is a predominately nuclear protein in GFP-only control cells (A) but frequently accumulated in the perinuclear region in cells expressing GA1020 (B), GR1136 (C) or PR1100 (D). The percentage of cells with Ran mislocalisation was increased by expression of both GA (P = 0.1226) and PR (P = 0.0025), however this was only statistically significant for GR Scale bars indicate 15 μm. (E) Quantification of Ran staining intensity in the nucleus compared to the perinuclear region. Ran was considered to be mislocalised if less than 90% of staining was nuclear. n = 3, with a minimum of 30 cells analysed for each independent replicate. Data was analysed by one-way ANOVA with Dunnett’s multiple comparison test. Error bars indicate SEM.
