Figure 6

LDH is crucial for glyoxylate metabolism. (A) Schematic of glyoxylate and its reactions relative to the TCA cycle. LDH is required for glyoxylate’s oxidation and conversion of pyruvate to lactate. It can also be used for the reduction of glyoxylate. (B) Survival of 5 dpf zebrafish 20 h after administration of combinations of the LDH inhibitor GSK2837808A, cyanide, and glyoxylate. Data are presented as mean ± SEM (****p < 0.0001 using one-way ANOVA). There was no significant difference between the untreated control and KCN + glyoxylate and no significant difference between inhibitor + KCN and KCN alone. Each condition had 3 fish per well, N = 3 wells for inhibitor alone, N = 8 for untreated control, N = 7 for KCN alone, N = 4 for KCN + glyoxylate, N = 8 for KCN + glyoxylate + inhibitor, N = 28 for KCN + inhibitor.