Figure 2 | Scientific Reports

Figure 2

From: Mycobacterium avium-intracellulare complex promote release of pro-inflammatory enzymes matrix metalloproteinases by inducing neutrophil extracellular trap formation

Figure 2

PAD4 mediates MAC-induced NET formation. (A) The effect of PAD4 inhibitor on the number of cit-H3 positive neutrophils. Data are means ± SD from five independent experiments. (B) The effect of PAD4 inhibitor on the expression levels of cit-H3. Representative protein band are shown. Molecular weights (kDa) are indicated on the left. H3 and β-actin were used as an internal control. Blots were cropped from different parts of the same gel. Original blots are presented in Supplementary Fig. 6. (C) Relative band intensities were calculated on the basis of densitometric analysis using ImageJ processing software. Protein levels of cit-H3 were calculated as the ratio of A-23187 pre-incubated with DMSO. Data are means ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way analysis of variance (ANOVA) with Bonferroni's multiple comparison test.

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