Figure 5
From: Nigratine as dual inhibitor of necroptosis and ferroptosis regulated cell death
Antioxidant activity of nigratine. DPPH reduction assay was conducted for 30 min at room temperature after the addition of a DPPH solution at 15.85 µM (in 90/10, vol/vol, methanol/water) in 96-well plate. Reduction of DPPH was determined by absorbance measurement at 517 nm. Tocopherol (vitamin E), a well-known antioxidant, and Fer-1, as lipophylic antioxidant, were used as a positive controls. Data are shown as the mean ± SD of three replicates.
