Figure 6
From: Nigratine as dual inhibitor of necroptosis and ferroptosis regulated cell death
Nigratine protects human bronchial organoids from necroptosis and ferroptosis. (a) Time-lapse microscopy and the associated quantifications of the measure of plasma membrane permeabilization using propidium iodide incorporation in human primary bronchial organoids stimulated with the mix of 40 μM Z-VAD, 100 ng/ml Human TNF-α and 5 µM BV-6 in presence or absence of 25 μM nigratine, 40 μM necrostatin-1 or 40 μM ferrostatin-1 for 15 h. Data are plotted as means + /− SEM. pValue calculated by t-test. (b) Time-lapse microscopy and the associated quantifications of the measure of plasma membrane permeabilization using propidium iodide incorporation in human primary bronchial organoids stimulated with 800 µM of cumene hydroperoxide in presence or absence of 25 μM nigratine, 40 μM necrostatin-1 or 40 μM ferrostatin-1 for 4 h. Data are plotted as means +/− SEM. pValues were calculated by t-test (**pValue < 0.01, ***pValue < 0.001, ****pValue < 0.0001).
