Table 2 The applied stressors of CUMS. CUMS; chronic unpredictable mild stress.
From: Serum metabolomic responses to aerobic exercise in rats under chronic unpredictable mild stress
Stressor | Description |
---|---|
Water deprivation | 24 h of water deprivation |
Food deprivation | 24 h of food deprivation |
Ice bath | Rats placed on glass jar with ice water at 4 °C to swim for 5 min |
Ultrasonic stimulation | Ultrasound stimulation (60 W) lasted for 3 h |
Thermal stimulus | Rats placed on electric thermostat at 45 °C for 10 min |
Electric shocks | Rats were placed in a plantar electric box with a voltage of 32 V, and the electric shock was given every 10 s for 2 s for a total of 10 times |
Tail clamp | The tail of the rats was clamped with a long tail clip for 2 min (the rats whined) |
Restraint | Rats was restrained in transparent plastic bottles for 3 h and returned to their original cage afterward |
Day and night reversal | Rats placed on the dark space during the day and in the bright space at night for 12 h each |