Figure 3
Heat treatment at 39 °C facilitates hepatic functions of hESC-HLCs. (A) Albumin (ALB) secretion from 37- to 39 °C-treated hESC-HLCs at 12 d.a.h. measured by ELISA. (B,C) Immunofluorescent micrographs (B) and single-cell profiling (C) of ALB in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). (D) α-fetoprotein (AFP) secretion from 37- to 39 °C-treated hESC-HLCs at 12 d.a.h. measured by ELISA. (E,F) Immunofluorescent micrographs (E) and single-cell profiling (F) of AFP in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). (G,H) Immunofluorescent micrographs (G) and single-cell profiling (H) of α1-anti trypsin (A1AT) in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). (I,J) Micrographs (I) and the percentiles of positive area (J) of 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h., stained with indocyanine green (ICG) (n = 5) Scale bars = 50 µm. (K,L) Immunofluorescent micrographs (K) and single-cell profiling (L) of organic anion transporting polypeptide 1B3 (OATP1B3) in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). DAPI was used for nuclei staining. Scale bars = 50 µm. In all panels, where applicable, center lines of box plots indicate medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001; each plot and error bar represents a mean ± SD.
