Figure 5
Evaluation of expression of collagen type I (COL1A), collagen type IV (COL4A), and collagen type III (COL3A) in hESC-HLCs with heat treatments. (A,B) Immunofluorescent micrographs (A) and single-cell profiling (B) of COL1A in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). (C,D) Immunofluorescent micrographs (C) and single-cell profiling (D) of COL4A in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). (E,F) Immunofluorescent micrographs (E) and single-cell profiling (F) of COL3A in 37 °C- and 39 °C-treated hESC-HLCs at 12 d.a.h. (n = 3). In all panels, where applicable, center lines of box plots indicate medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; ****p < 0.0001; each plot and error bar represents a mean ± SD. DAPI was used for nuclei staining. Scale bars = 50 µm.
