Figure 3
Activity of TMPRSS2 in the presence of AAT alone or with UFH, nadroparin, or enoxaparin. HEK293TTMPRSS2 cells were pre-treated with AAT alone at the indicated concentrations or with (A) UFH, (B) nadroparin (Nadro), or (C) enoxaparin (Enox) at the indicated final concentrations for 30 min, followed by addition of the fluorescent substrate Boc-QAR-AMC. Fluorescence was measured immediately on the fluorescent plate reader and then every 15 min for a total of 90 min. (D) TMPRSS2 activity at 90 min in untreated cells and cells incubated with AAT ± two concentrations of nadroparin, UFH, and enoxaparin. Data shown are the triplicate means ± SEM of three independent experiments. *p < 0.05, **p < 0.01 at 90 min. Unfractionated heparin = UFH.
