Figure 8

Validation of RNASeq data with qPCR. (a) and (b) Expression of five randomly selected genes was examined by qPCR analysis. For each gene, fold changes were calculated by ΔΔCt method and log2Fold change were compared between qPCR and RNAseq. (c) Correlation between RNAseq and qPCR data based on log2fold change of the five selected genes: y = 0.31x – 0.8.