Figure 4

Standard curve generated from the amplification of nine dilutions of standard DNA in the range from one to 108 DNA copies per one μL; each dilution was run in triplicates and standard deviation for each dilution was calculated and displayed. The standard curve served for the quantification of initial copy numbers of T. gondii B1 gene in investigated wastewater and water samples collected from the area of Qinghai Province, P.R. China. Figure was prepared using MATLAB R2015a.