Table 2 Characteristics of the positive water samples collected from different water sources in Qinghai Province, P.R. China, regarding sampling time, reproducibility of real-time PCR results and the determined Toxoplasma genotype.

From: Investigation of Toxoplasma gondii in wastewater and surface water in the Qinghai-Tibet Plateau, China using real-time PCR and multilocus genotyping

Template No

Sampling site

Sampling month

Microscopy

Reproducibility of real-time PCR

qPCR results

Type

Cq (ΔR) av

Cq (ΔR) SD

NP

OCS

SCS

CCS

67

Xining WWTP

April

–

3/3

30.3

0.1

1.19e + 002

4

8

34

I

83

Baide slaughterhouse

April

–

3/3

27.7

0.06

7.00e + 002

25

50

200

I

120

Huangyuan WWTP

August

–

3/3

26.3

0.13

1.84e + 003

65

130

520

I

125

Huangyuan WWTP

August

–

3/3

24.1

0.03

8.27e + 003

295

590

2360

ND

  1. (3/3) three positive results obtained per three repeats of real-time PCR.
  2. ND not determined, Cq (ΔR) av. average Cq values obtained from triplicates of positive water samples, Cq (ΔR) SD values of standard deviation obtained from triplicates of positive water samples, NP initial copy number (number of copies of T. gondii B1 gene) present in positive water samples calculated based on the standard curve, OCS the equivalent of the initial number of T. gondii oocysts present in positive water samples. SCS – the equivalent of the initial number of T. gondii sporocysts present in positive water samples (OCS × 2, one oocyst consist of two sporocysts). CCS equivalent of initial number of T. gondii cells (sporozoites) present in positive water samples (OCS × 8, one oocyst consist of two sporocysts filled with four sporozoites each).
Back to article page