Table 3 Pathways affected by hypermethylation of genes in cloned relative to IVF at 2-cell, 8-cell and blastocyst stage embryos.

2-cell stage

Integrin signalling pathway (P00034)

7.13E−05

5.95E−03

Inflammation mediated by chemokine and cytokine signaling pathway (P00031)

5.62E−04

3.13E−02

Gonadotropin-releasing hormone receptor pathway (P06664)

8.59E−04

3.59E−02

PDGF signaling pathway (P00047)

2.15E−03

7.20E−02

CCKR signaling map (P06959)

2.28E−03

6.35E−02

Endothelin signaling pathway (P00019)

4.51E−03

1.08E−01

Apoptosis signaling pathway (P00006)

5.45E−03

1.14E−01

Ras Pathway (P04393)

1.13E−02

2.10E−01

Angiogenesis (P00005)

1.36E−02

2.27E−01

Wnt signaling pathway (P00057)

1.56E−02

2.36E−01

T cell activation (P00053)

2.19E−02

3.04E−01

EGF receptor signaling pathway (P00018)

2.35E−02

3.02E−01

B cell activation (P00010)

2.63E−02

3.14E−01

Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway (P00027)

3.82E−02

4.25E−01

Histamine H1 receptor mediated signaling pathway (P04385)

3.90E−02

4.07E−01

8-cell stage

Angiogenesis (P00005)

4.38E−04

3.65E−02

Integrin signalling pathway (P00034)

5.97E−04

3.33E−02

PDGF signaling pathway (P00047)

1.99E−03

8.32E−02

CCKR signaling map (P06959)

4.36E−03

1.46E−01

Wnt signaling pathway (P00057)

8.09E−03

2.25E−01

Gonadotropin-releasing hormone receptor pathway (P06664)

1.27E−02

3.02E−01

Alzheimer diseasE−presenilin pathway (P00004)

1.59E−02

3.32E−01

Ras Pathway (P04393)

1.98E−02

3.67E−01

EGF receptor signaling pathway (P00018)

3.11E−02

5.19E−01

Endothelin signaling pathway (P00019)

3.49E−02

5.30E−01

Apoptosis signaling pathway (P00006)

3.63E−02

5.05E−01

Oxytocin receptor mediated signaling pathway (P04391)

4.03E−02

5.17E−01

PI3 kinase pathway (P00048)

4.48E−02

5.34E−01

Oxidative stress response (P00046)

4.48E−02

4.99E−01

5HT2 type receptor mediated signaling pathway (P04374)

4.72E−02

4.93E−01

Blastocyst stage

Inflammation mediated by chemokine and cytokine signaling pathway (P00031)

8.20E−05

6.85E−03

Wnt signaling pathway (P00057)

2.71E−04

1.51E−02

Endothelin signaling pathway (P00019)

4.57E−04

1.91E−02

Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway (P00027)

4.92E−04

1.64E−02

Integrin signalling pathway (P00034)

9.68E−04

2.69E−02

Blood coagulation (P00011)

2.10E−03

5.00E−02

Nicotinic acetylcholine receptor signaling pathway (P00044)

2.52E−03

5.25E−02

PDGF signaling pathway (P00047)

3.24E−03

6.02E−02

Angiogenesis (P00005)

4.10E−03

6.85E−02

DNA replication (P00017)

5.33E−03

8.09E−02

Ionotropic glutamate receptor pathway (P00037)

5.61E−03

7.81E−02

Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway (P00026)

7.23E−03

9.29E−02

Alzheimer diseasE−amyloid secretase pathway (P00003)

8.11E−03

9.67E−02

EGF receptor signaling pathway (P00018)

1.19E−02

1.32E−01

Nicotine pharmacodynamics pathway (P06587)

1.28E−02

1.34E−01

Gonadotropin-releasing hormone receptor pathway (P06664)

1.31E−02

1.29E−01

Metabotropic glutamate receptor group I pathway (P00041)

1.37E−02

1.27E−01

Alzheimer disease-presenilin pathway (P00004)

1.44E−02

1.27E−01

Histamine H1 receptor mediated signaling pathway (P04385)

1.57E−02

1.31E−01

Metabotropic glutamate receptor group III pathway (P00039)

1.98E−02

1.58E−01

Cadherin signaling pathway (P00012)

3.19E−02

2.42E−01

5HT2 type receptor mediated signaling pathway (P04374)

3.34E−02

2.42E−01

Axon guidance mediated by netrin (P00009)

3.51E−02

2.44E−01

Oxytocin receptor mediated signaling pathway (P04391)

3.64E−02

2.43E−01

Insulin/IGF pathway-protein kinase B signaling cascade (P00033)

4.45E−02

2.86E−01

B cell activation (P00010)

4.73E−02

2.92E−01

Plasminogen activating cascade (P00050)

4.94E−02

2.95E−01