Table 1 Primer pairs and PCR efficiencies used for RT-PCR and qRT-PCR in this study.
From: Newcastle disease virus genotype VII gene expression in experimentally infected birds
Gene symbol | Primer sequence | Fragment size (bp) | Ta °C | Efficiency |
|---|---|---|---|---|
NP | Forward: ATGAGAGCAGTGGCGAACAG | 112 | 60 | 1.94 |
Reverse: CCCAGTCAGTGTCGTTGTCT | ||||
P | Forward: CATCCTTAAGTGATCTCCGA | 123 | 54 | 1.99 |
Reverse: CCGGTTGTGAGAGTTTATTG | ||||
M | Forward: CTGCATATCGGGCTTATGTCCACT | 112 | 62 | 1.93 |
Reverse: GCACATCACTGAGCCCAACAGATA | ||||
F | Forward: AAGCTCTCTTGATGGCAGGC | 91 | 58 | 1.99 |
Reverse: CCCTGTTTGAGACGAGGTGT | ||||
HN | Forward: GGACATCTGCAACAGGGAGG | 86 | 61 | 1.93 |
Reverse: CACACTGCAGGACTTCCGAT | ||||
L | Forward: GCATCCACTGTAGCACGACTATGT | 128 | 62 | 1.98 |
Reverse: GGTTGCGAGCTGTGGGTAATAGAA |
